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A flow cytometry method for rapid detection and enumeration of total bacteria in milk


, : A flow cytometry method for rapid detection and enumeration of total bacteria in milk. Applied and environmental microbiology 66(3): 1228-1232

Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (< or = 1-h) FCM assay based on enzymatic clearing of milk to determine total bacteria in milk. When bacteria were added to ultra-heat-treated milk, a good correlation (r > or = 0.98) between the FCM assay and the more conventional methods of plating and direct microscopic counting was achieved. Raw milk data showed a significant correlation (P < 0.01) and a good agreement (r = 0.91) between FCM and standard plate count methods. The detection limit of the FCM assay was < or = 10(4) bacteria ml of milk(-1). This limit is below the level of detection required to satisfy legislation in many countries and states.

Accession: 003342912

PMID: 10698799

DOI: 10.1128/aem.66.3.1228-1232.2000

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