A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle
Sreevatsan, S.; Bookout, J.B.; Ringpis, F.; Perumaalla, V.S.; Ficht, T.A.; Adams, L.G.; Hagius, S.D.; Elzer, P.H.; Bricker, B.J.; Kumar, G.K.; Rajasekhar, M.; Isloor, S.; Barathur, R.R.
Journal of Clinical Microbiology 38(7): 2602-2610
A multiplex amplification and detection method for the diagnosis of M. bovis and B. abortus infection simultaneously in bovine milk and nasal secretions (bovine pathogen detection assay PCR) was based on duplex amplification of species-specific targets (a region of the BCSP31K gene of B. abortus and a repeat-sequence region in the hsp65 gene of M. bovis, respectively) followed by a solid-phase probe capture hybridization of amplicons for detection.