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A rapid, automated enzymatic fluorometric assay for determination of D-arabinitol in serum



A rapid, automated enzymatic fluorometric assay for determination of D-arabinitol in serum



Journal of Clinical Microbiology 38(4): 1439-1443



A rapid enzymatic fluorometric assay for measuring D-arabinitol in serum was developed using recombinant D-arabinitol dehydrogenase from Candida albicans (rArDH). rArDH was produced in Escherichia coli and purified by dye-ligand affinity chromatography. rArDH was highly specific for D-arabinitol, cross-reacting only with xylitol (4.9%) among all polyols tested.

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Accession: 003347002

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PMID: 10747122


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