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A viral transmembrane recombinant protein-based enzyme-linked immunosorbent assay for the detection of bovine immunodeficiency virus infection

Abed, Y.; Archambault, D.

Journal of Virological Methods 85(1-2): 109-116

2000


ISSN/ISBN: 0166-0934
PMID: 10716344
DOI: 10.1016/s0166-0934(99)00161-5
Accession: 003349653

An ELISA based on bovine immunodeficiency virus (BIV) truncated transmembrane envelope protein (tTM) was developed for the serodetection of BIV infection. 109 bovine sera (86 BIV-negative and 23 BIV-positive) were tested. The ELISA results were compared with those of 3 Western blot assays using, as test antigens, cell culture-derived whole virus proteins (WB1) and the tTM (WB2) and p26 (WB3) fusion proteins expressed from recombinant baculovirus in insect cells, respectively.

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