+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Application of PCR assays to determine the genotype of Babesia bovis parasites isolated from cattle with clinical babesiosis soon after vaccination against tick fever

Application of PCR assays to determine the genotype of Babesia bovis parasites isolated from cattle with clinical babesiosis soon after vaccination against tick fever

Australian Veterinary Journal 78(3): 179-181

To demonstrate the value of PCR assays to determine the genotypes of Babesia bovis in cattle with clinical signs of babesiosis within 3 weeks after vaccination against tick fever. Samples from 5 cases of babesiosis in cattle soon after vaccination against tick fever were analysed in two PCR assays. Parasite DNA was purified from blood taken from cattle with signs of babesiosis within 3 weeks of vaccination against tick fever. DNA was also prepared from the tissues of animals that died of babesiosis. Two PCR assays that amplify repeat sequences of DNA within the B bovis genes, Bv80 and BvVA1, were used to differentiate the genotypes of field isolates and vaccine strains of B bovis. One of the five cases of babesiosis was found to be caused by a vaccine strain, but PCR analyses showed that the predominant isolate in the other four cases was not the vaccine strain. PCR assays on the DNA of B bovis obtained from the blood or tissues of cattle clinically affected with tick fever within 3 weeks after vaccination are useful to distinguish between vaccine strains and field isolates as the source of infection.

(PDF emailed within 0-6 h: $19.90)

Accession: 003362871

Download citation: RISBibTeXText

PMID: 10860157

DOI: 10.1111/j.1751-0813.2000.tb10588.x

Related references

Bovine babesiosis: vaccination against tick-borne challenge exposure with culture-derived Babesia bovis immunogens. American Journal of Veterinary Research 40(12): 1678-1682, 1979

Genotypic diversity in field isolates of Babesia bovis from cattle with babesiosis after vaccination. Australian Veterinary Journal 75(8): 575-578, 1997

Comparison between conventional and molecular methods for diagnosis of bovine babesiosis ( Babesia bovis infection ) in tick infested cattle in upper Egypt. Journal of Parasitic Diseases 41(1): 243-246, 2017

Growth of Babesia bovis parasites in stationary and suspension cultures and their use in experimental vaccination of cattle. Research in Veterinary Science 47(3): 309-314, 1989

Cell-mediated immune responses to Babesia bovis merozoite antigens in cattle following infection with tick-derived or cultured parasites. Infection and Immunity 59(7): 2418-2426, 1991

Detection of tick blood parasites in Egypt using PCR assay I--Babesia bovis and Babesia bigemina. Parasitology Research 105(3): 721-730, 2009

Prevalence of babesiosis (Babesia bovis and Babesia bigemina) in cattle and water buffalo in Nueva Ecija, Philippines using Nested Polymerase Chain Reaction. Annals of Parasitology 63(4): 309-316, 2018

Bovine babesiosis: Cattle protected in the field with a frozen vaccine containing Babesia bovis and Babesia bigemina cultured in vitro with a serum-free medium. Parasitology International 67(2): 190-195, 2017

The epidemiology of babesiosis in cattle with special reference to the Babesia bovis. Haemoprotozoan diseases of domestic animals: proceedings Seminar CwVA Asian Australian regions Haryana Agricultural Univ Hissar India Oct 27 Nov 1-1980 edited by OP Gautam RD Sharma S Dhar: 106, 1980

Attempts to adapt the cattle parasites babesia bovis and babesia bigemina in the syrian hamster. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 75, 1990

Seroprevalence of Babesia bovis and Babesia bigemina in cattle in the Soutpansberg region, Limpopo Province, South Africa, associated with changes in vector-tick populations. Journal of the South African Veterinary Association 77(2): 61-65, 2006

An outbreak of babesiosis (Babesia bovis) in a beef cattle fattening unit. Obiettivi e Documenti Veterinari 21(3): 65-70, 2000

Use of cerebellar brain smears in the diagnosis of babesiosis (Babesia bovis) in cattle. Tropical Animal Health and Production 14(4): 242-246, 1982

Serological responses to Babesia bovis vaccination in cattle previously infected with Babesia bigemina. Veterinary Parasitology 170(1-2): 30-36, 2010

Use of a duplex PCR/DNA probe assay to monitor Babesia bovis and Babesia bigemina in cattle during a vaccination trial. Revista Latinoamericana de Microbiologia 40(1-2): 39-44, 2000