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Application of Western Blot to detect bovine leukaemia virus infections using recombinant p24 antigen

Application of Western Blot to detect bovine leukaemia virus infections using recombinant p24 antigen

Medycyna Weterynaryjna 57(8): 586-590

The article describes the Western Blot assay used in detecting antibodies for bovine leukaemia virus (BLV) p24 antigen (WB/p24). The test employs a bacterially synthesized p24 antigen which represents a fusion protein consisting of thioredoxin and the mature viral p24. This assay is compared with two other techniques for detecting either total (ELISA/BLV) and anti-p24 and gp51-related antibodies (Western Blot/BLV) in sheep experimentally infected as well as cattle naturally infected with BLV.

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Accession: 003362904

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