Assessing chromosomal abnormalities in two-cell bovine in vitro-fertilized embryos by using fluorescent in situ hybridization with three different cloned probes
Slimane, W.; Heyman, Y.; Lavergne, Y.; Humblot, P.; Renard, J.P.
Biology of Reproduction 62(3): 628-635
ISSN/ISBN: 0006-3363 PMID: 10684804 DOI: 10.1095/biolreprod62.3.628
The efficiency of fluorescent in situ hybridization (FISH) for detecting chromosomal abnormalities in in vitro-fertilized (IVF) bovine embryos as early as the 2-cell stage was investigated. Three different cloned probes were used, 2 derived from a unique sequence specific to the subtelomeric (D1S48) or subcentromeric regions (19C10) of chromosome 1 and the 3rd (H1A clone) derived from a repetitive sequence that hybridizes to the subcentromeric regions of 3 other chromosomes (14, 20 and 25).