+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection



Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection



Journal of Dairy Science 84(5): 1077-1084



Staphylococcus aureus is a major pathogen in bovine intramammary infections of subclinical and chronic nature. Persistent infection with S. aureus has been postulated to be associated with an impaired immune response. This study was designed to define changes in peripheral blood and milk cell subpopulations during chronic S. aureus infection. The expression of specific antigens on the surface of lymphocytes and neutrophils was studied by flow cytometry. Cytokines and cytokine transcripts elaborated by the milk-derived cells were also investigated, using ELISA and reverse transcription polymerase chain reaction, respectively. The results indicated that cell subpopulations in blood from infected cows were not modified. In contrast, changes occurred in infected milk: neutrophils were the main cell population, but they were not in a highly activated state; the CD8+ T-lymphocytes were mainly recruited compared with the CD4+ T-lymphocytes, suggesting that CD8+ T-lymphocytes play an important role in chronic S. aureus infection. Also, the proportion of the B-lymphocytes among the total lymphocyte population was increased, suggesting that a humoral response developed, and no change was observed in the gammadelta subset. No cytokine mRNA was found in milk cells from uninfected mammary glands. In contrast, interleukin (IL)-1alpha, IL-1beta, IL-6 and tumor necrosis factor alpha pro-inflammatory cytokine and IL-10 and IL-12 regulatory cytokine mRNA were synthesized in cells derived from infected mammary glands, whereas no IL-2 nor IL-4 mRNA were found. Therefore, cells present in milk during chronic S. aureus infection were activated, but did not reveal any polarization of the immune response.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003377045

Download citation: RISBibTeXText

PMID: 11384034

DOI: 10.3168/jds.s0022-0302(01)74568-7


Related references

MicroRNA expression profiles of bovine milk exosomes in response to Staphylococcus aureus infection. Bmc Genomics 16: 806, 2015

Nonspecific cell mediated immune response in mice following chronic infection with staphylococcus aureus. Abstracts of the Annual Meeting of the American Society for Microbiology 75: 77, 1975

Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells. Bmc Genomics 13: 540, 2012

Cytokine gene expression in an experimental animal model of bacteremic Staphylococcus aureus infection. FASEB Journal 10(6): A1433, 1996

The SaeR/S gene regulatory system induces a pro-inflammatory cytokine response during Staphylococcus aureus infection. Plos one 6(5): E19939, 2011

Characterization of cytokine expression in milk somatic cells during intramammary infections with Escherichia coli or Staphylococcus aureus by real-time PCR. Veterinary Research 37(2): 219-229, 2006

Cytokine response in patients with chronic infections caused by Staphylococcus aureus strains and diversification of their Agr system classes. European Journal of Clinical Microbiology and Infectious Diseases 31(10): 2809-2815, 2012

Chloroquine differentially modulates inflammatory cytokine expression in RAW 264.7 cells in response to inactivated Staphylococcus aureus. Inflammation 38(2): 745-755, 2015

Correlation of histopathologic and bacteriologic changes with cytokine expression in an experimental murine model of bacteremic Staphylococcus aureus infection. Infection and Immunity 65(9): 3889-3895, 1997

Innate immune response of corneal epithelial cells to Staphylococcus aureus infection: role of peptidoglycan in stimulating proinflammatory cytokine secretion. Investigative Ophthalmology & Visual Science 45(10): 3513-3522, 2004

Effect of a milk-derived factor on the inflammatory response to Staphylococcus aureus intramammary infection. Veterinary Immunology and Immunopathology 30(2-3): 233-246, 1992

Modulation of cytokine production from an EpiOcular corneal cell culture model in response to Staphylococcus aureus superantigen. Australian and new Zealand Journal of Ophthalmology 25(Suppl. 1): S43-S45, 1997

Murine immune response to a chronic Staphylococcus aureus biofilm infection. Infection and Immunity 79(4): 1789-1796, 2011

Characterization of the humoral immune response during Staphylococcus aureus bacteremia and global gene expression by Staphylococcus aureus in human blood. Plos one 8(1): E53391, 2013

Alpha-MSH reduces the internalization of Staphylococcus aureus and down-regulates HSP 70, integrins and cytokine expression in human keratinocyte cell lines. Experimental Dermatology 13(12): 748-754, 2004