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Characterization of expressed sequence tags generated from skin cDNA clones of Equus caballus by single pass sequencing



Characterization of expressed sequence tags generated from skin cDNA clones of Equus caballus by single pass sequencing



Animal Biotechnology 12(1): 87-97



A cDNA library was built using RNA extracted from the skin tissue of an adult horse. The library was primed with oligo (dT) and sequences were directionally inserted in order to produce an expression library. The library has 5.8X 105 plaque forming units with 99.6% recombinant phage. The average insert size is 1.3 Kbp. Three hundred and thirteen expressed sequence tags (ESTs) were generated from sequencing of the 5 prime end of randomly selected skin cDNA clones.

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Accession: 003380338

Download citation: RISBibTeXText

PMID: 11370684

DOI: 10.1081/ABIO-100102981


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