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Characterization of nuclear microsatellites in Pinus halepensis Mill. and their inheritance in P. halepensis and Pinus brutia Ten



Characterization of nuclear microsatellites in Pinus halepensis Mill. and their inheritance in P. halepensis and Pinus brutia Ten



Molecular Ecology 9(12): 2157-2159



A microsatellite library enriched for di- (GC, CT, CA), tri- (CAA, GCC) and tetra-nucleotide (GATA, CATA) repeats was constructed for Pinus halepensis. A total of 43 clones containing a microsatellite were detected from 47 clones randomly chosen from the library. Total genomic DNA extracted from leaf and megagametophyte tissue was used for testing of primer pairs. Out of 25 primer pairs, nine (36%) either gave no amplification (n = 4) or produced multibanded patterns (n = 5). Sixteen produced fragment amplification in the expected size range, of which 8 were polymorphic within one or the other species. nSSR polymorphism was screened at the population level using 50 P. brutia individuals from 2 populations and 47 P. halepensis individuals from 3 populations. The maximum number of alleles per locus was 4 and expected heterozygosity per locus was between 0.479 and 0.676, lower than expected from studies in other conifers but greater than that found using isoenzymes.

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Accession: 003380539

Download citation: RISBibTeXText

PMID: 11123630

DOI: 10.1046/j.1365-294x.2000.10532.x


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