Section 4
Chapter 3,383

Chitin as a measure of biomass of Crinipellis perniciosa, causal agent of witches' broom disease of Theobroma cacao

Penman, D.; Britton, G.; Hardwick, K.; Collin, H.A.; Isaac, S.

Mycological Research 104(6): 671-675


ISSN/ISBN: 0953-7562
DOI: 10.1017/s0953756299001689
Accession: 003382300

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Crinipellis perniciosa is the cause of witches' broom disease of cocoa, a serious problem in South America. The aim of the project was to develop a measure of fungal biomass in cocoa tissue infected with C. perniciosa using chitin as a marker. Axenic cultures of primary and secondary mycelium of C. perniciosa were exposed to strong hydrochloric acid to hydrolyse the chitin.

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