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Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3



Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3



Biochimica et Biophysica Acta, Molecular and Cell Biology of Lipids 1487(1): 106-111



A cotton Ltp3 gene and its 5' and 3' flanking regions have been cloned with a PCR-based genomic DNA walking method. The amplified 2.6 kb DNA fragment contains sequences corresponding to GH3 cDNA which has been shown to encode a lipid transfer protein (LTP3). The gene has an intron of 80 bp which is located in the region corresponding to the C-terminus of LTP3. The Ltp3 promoter was systematically analyzed in transgenic tobacco plants by employing the Escherichia coli beta-glucuronidase gene (GUS) as a reporter. The results of histochemical and fluorogenic GUS assays indicate that the 5' flanking region of the Ltp3 gene contains cis-elements conferring the trichome specific activity of Ltp3 promoter.

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Accession: 003384725

Download citation: RISBibTeXText

PMID: 11004611

DOI: 10.1016/s1388-1981(00)00072-x


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