Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

Bart-Delabesse, E.; Sarfati, J.; Debeaupuis, J.P.; van Leeuwen, W.; van Belkum, A.; Bretagne, S.; Latge, J.P.

Journal of Clinical Microbiology 39(7): 2683-2686


ISSN/ISBN: 0095-1137
PMID: 11427596
DOI: 10.1128/jcm.39.7.2683-2686.2001
Accession: 003389232

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Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.