+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of virus isolation and reverse transcription polymerase chain reaction assay for detection of bovine viral diarrhea virus in bulk milk tank samples



Comparison of virus isolation and reverse transcription polymerase chain reaction assay for detection of bovine viral diarrhea virus in bulk milk tank samples



Journal of Veterinary Diagnostic Investigation 12(2): 184-186



Over 3 years, 144 samples from 97 farms were tested for BVDV using an RT-PCR assay in conjunction with a classical virus isolation (VI) procedure. Virus could be detected with both methods when the milk from a single persistently infected animal was diluted 1:600 with the milk from a herd of BVDV-negative animals. Based on individual farms, there was an overall prevalence of 12.4% BVDV infection, and the correlation between the 2 assays was 95.9%.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003389800

Download citation: RISBibTeXText

PMID: 10730955

DOI: 10.1177/104063870001200219


Related references

Detection of the genome of bovine viral diarrhea virus (BVDV) using the polymerase chain reaction after reverse transcription (RT-PCR): comparison of methods for the isolation of ribonucleic acid (RNA) from clinical samples. Dtw. Deutsche Tierarztliche Wochenschrift 107(11): 431-437, 2000

Detection of bovine viral diarrhea virus (BVDV) using reverse transcription polymerase chain reaction assay. Journal of Veterinary Medical Science 60(7): 867-870, 1998

Detection of Bovine Viral Diarrhea Virus(Bvdv) Using Reverse Transcription Polymerase Chain Reaction Assay. Journal of Veterinary Medical Science 60(7): 867-870, 1998

Evaluation of skin samples for bovine viral diarrhea virus by use of reverse transcriptase polymerase chain reaction assay after vaccination of cattle with a modified-live bovine viral diarrhea virus vaccine. American Journal of Veterinary Research 73(2): 319-324, 2012

Detection of bovine diarrhoea virus (BDV) using the polymerase chain reaction after reverse transcription (RT-PCR): comparison of methods for the isolation of RNA from clinical samples. Deutsche Tieraerztliche Wochenschrift 107(11): 431-437, 2000

Detection of bovine viral diarrhea virus by TaqMan reverse transcription polymerase chain reaction. Journal of Veterinary Diagnostic Investigation 14(2): 120-125, 2002

Detection of Bovine Viral Diarrhea Virus by TaqMan(R) Reverse Transcription Polymerase Chain Reaction. Journal of Veterinary Diagnostic Investigation 14(2): 120-125, 2002

Sensitivity of polymerase chain reaction for detection of bovine viral diarrhea virus in pooled serum samples and use of pooled polymerase chain reaction to determine prevalence of bovine viral diarrhea virus in auction market cattle. Journal of Veterinary Diagnostic Investigation 20(1): 75-78, 2008

Combination of reverse transcription real-time polymerase chain reaction and antigen capture enzyme-linked immunosorbent assay for the detection of animals persistently infected with Bovine viral diarrhea virus. Journal of Veterinary Diagnostic Investigation 23(1): 16-25, 2011

A comparison of polymerase chain reaction with and without RNA extraction and virus isolation for detection of bovine viral diarrhea virus in young calves. Journal of Veterinary Diagnostic Investigation 14(5): 433-437, 2002

Reverse transcription-polymerase chain reaction on pooled samples to detect bovine viral diarrhea virus by using fresh ear-notch-sample supernatants. Journal of Veterinary Diagnostic Investigation 18(1): 89-93, 2006

Comparison of a reverse transcription-polymerase chain reaction assay and virus isolation for the detection of classical swine fever virus. Revue Scientifique et Technique 17(3): 674-681, 1998

Detection by reverse transcription-polymerase chain reaction of genes for the Bovine viral diarrhea virus in formalin-fixed, paraffin-embedded cattle tissues. Journal of the Japan Veterinary Medical Association 55(10): 652-657, 2002

Effect of temperature on the detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples by reverse transcription-polymerase chain reaction. Journal of Veterinary Diagnostic Investigation 16(3): 237-239, 2004

Applications of a duplex reverse transcription polymerase chain reaction and direct immunofluorescence assay in comparison with virus isolation for detection of influenza A and B. Diagnostic Microbiology and Infectious Disease 57(1): 53-57, 2007