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Construction of recombinant eukaryotic expression vector of nucleocapsid gene of avian infectious bronchitis virus


Construction of recombinant eukaryotic expression vector of nucleocapsid gene of avian infectious bronchitis virus



Chinese Journal of Veterinary Science 21(1): 14-17



Nucleocapsid (N) Gene cDNA of isolate HB strain was amplified by RT-PCR and cloned into KpnI site of plasmid pUC119. It was sequenced and subcloned into KpnI site of plasmid pcDNA3. The recombinant plasmid pcDNA-N was identified by KpnI, PCR and southern hybrization.

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