DNA polymorphism of Mycobacterium abscessus analyzed by infrequent-restriction-site polymerase chain reaction
Su, L.H.; Chia, J.H.; Leu, H.S.; Cheng, S.W.; Kuo, A.J.; Sun, C.F.; Wu, T.L.
Chang Gung Medical Journal 23(8): 467-475
ISSN/ISBN: 2072-0939 PMID: 11039248 Accession: 003397886
Mycobacterium abscessus is an important pathogen that has been increasingly associated with many clinical and nosocomial infections. A reliable molecular typing scheme is essential for the epidemiological study of this rapidly growing mycobacterium. Pulsed-field gel electrophoresis (PFGE), considered to be the gold standard among molecular typing methods, has failed to provide satisfactory results in the molecular typing of this bacterium. A newly developed molecular typing method, infrequent-restriction-site polymerase chain reaction (IRS-PCR), was examined in this study to determine its suitability for fingerprinting M. abscessus isolates.