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Detection of Chrysanthemum stunt viroid and Potato spindle tuber viroid by return-polyacrylamide gel electrophoresis



Detection of Chrysanthemum stunt viroid and Potato spindle tuber viroid by return-polyacrylamide gel electrophoresis



Bulletin OEPP 30(3/4): 453-456



Return-polyacrylamide gel electrophoresis (R-PAGE) is one of the methods used for the detection of viroids, in particular chrysanthemum stunt viroid (CSVd) and potato spindle tuber viroid (PSTVd). The method is based on temperature-related conformational changes of the circular single-stranded RNA molecule. This results in a characteristic electrophoretic behaviour, providing a clear separation between the viroid and nucleic acids of the plant.

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Accession: 003400667

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DOI: 10.1111/j.1365-2338.2000.tb00928.x


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