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Detection of leaf-associated fungi based on PCR and nucleotide sequence of the ribosomal internal transcribed spacer (ITS) in Miscanthus



Detection of leaf-associated fungi based on PCR and nucleotide sequence of the ribosomal internal transcribed spacer (ITS) in Miscanthus



Botanical Bulletin of Academia Sinica 42(1): 39-44



PCR amplification of the internal transcribed spacer region (ITS) of the nuclear ribosomal DNA from the genomic DNA isolated from the leaf tissue of Miscanthus sinensis varieties and M. floridulus resulted in two amplified fragments. Via FASTA search, the shorter length of about 500 base pairs was identified as the ITS region of fungi symbiotic in the grass foliage.

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