Development and validation of an enzyme immunoassay for testosterone: effects of photoperiod on plasma testosterone levels and gonadal development in male sea bass (Dicentrarchus labrax, L.) at puberty
Rodriguez, L.; Begtashi, I.; Zanuy, S.; Carrillo, M.
Fish Physiology and Biochemistry 23(2): 141-150
ISSN/ISBN: 0920-1742 DOI: 10.1023/a:1007871604795
A specific immunoassay was developed for the quantification of testosterone (T) in sea bass plasma. Specific primary antibody against T was produced using an antigen BSA conjugated with T. The enzyme immunoassay (EIA) had a sensitivity of 5-0.009 ng ml-1 and 6.2% intra-assay variation; inter-assay variation was 9.5% for sea bass plasma.