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Development of cloned swamp buffalo embryos derived from fetal fibroblasts: comparison in vitro cultured with or without buffalo and cattle oviductal epithelial cells



Development of cloned swamp buffalo embryos derived from fetal fibroblasts: comparison in vitro cultured with or without buffalo and cattle oviductal epithelial cells



Buffalo Journal 15(3): 371-384



Swamp buffalo fetal fibroblasts were cultured in medium supplemented 10% fetal calf serum (FCS) before used as donor cells. In vitro matured buffalo oocytes were enucleated and used as recipient cytoplasts. Donor cells were transferred into cytoplasts by micromanipulation and fusion by electrostimulation. The overall fusion rate was 88.1% (245/278). 87.3% (214/245) and 65.3% (160/245) of reconstructed embryos cleaved and developed to the 8-cell stage, respectively.

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Accession: 003403982

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