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Differentiation of aflatoxin-producing and non-producing strains of Aspergillus flavus group



Differentiation of aflatoxin-producing and non-producing strains of Aspergillus flavus group



Letters in Applied Microbiology 33(4): 291-295



Three conventional methods and a multiplex PCR procedure with a set of four primers (Quadruplex-PCR) were used to differentiate between aflatoxin-producing and non-producing strains of the Aspergillus flavus group. By combining sets of primers for aflR, nor-1, ver-1 and omt-A genes of the aflatoxin biosynthetic pathway, Quadruplex-PCR showed that aflatoxinogenic strains gave a quadruplet pattern, indicating the presence of all the genes involved in the aflatoxin biosynthetic pathway which encode for functional products. Non-aflatoxinogenic strains gave varying results with one, two, three or four banding patterns. A banding pattern in three non-aflatoxinogenic strains resulted in non-differentiation between these and aflatoxinogenic strains. Because conventional methods are time-consuming, further studies are needed to develop a rapid and objective technique that permits complete differentiation between aflatoxin-producing and non-producing strains of the A. flavus group.

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Accession: 003407373

Download citation: RISBibTeXText

PMID: 11559403

DOI: 10.1046/j.1472-765x.2001.00998.x


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