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Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

International Journal of Systematic Bacteriology 49 Pt 4: 1907-1913

Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Genetic variation among 35 strains representing the four currently recognized species of Saccharomyces s.s. (Saccharomyces cerevisiae, S. bayanus, S. pastorianus/carlsbergensis and S. paradoxus) was estimated by analysing the electrophoretic mobilities of nonspecific esterases, acid phosphatase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase isoenzymes. 22 electrophoretic types were identified, a result in agreement with the phenotypic and genetic polymorphisms reported for this group of yeasts. However, the 4 species were clearly distinguishable based on the patterns obtained using 3 of the enzymes assayed, the resolving power not being improved by the introduction of data correspondent to lactate dehydrogenase. The overall diversity was higher among S. cerevisiae isolates, in contrast with S. paradoxus which showed only 2 patterns, one of which was common to 4 of the 5 strains studied. Concordant results from the application of the method and DNA hybridization experiments demonstrate its value for identification purposes.

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Accession: 003408813

PMID: 10555375

DOI: 10.1099/00207713-49-4-1907

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