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Effect of DNA quality on randomly amplified polymorphic DNA-based identification of strawberry (Fragaria x ananassa) genotypes






Journal of Fruit and Ornamental Plant Research 8(3/4): 107-115

Effect of DNA quality on randomly amplified polymorphic DNA-based identification of strawberry (Fragaria x ananassa) genotypes

Genomic DNA from strawberry callus was obtained using 5 extraction methods. DNA yield ranged from 70 to 220 micro g/g of the fresh tissue, depending on the procedure used, being highest with method I and V (220 and 160 micro g/g FW, respectively). Lower amounts of DNA were noted in the preparation derived from methods III and IV (~110 micro g/g FW) and the lowest after using the simple and shortest (~15 minutes) method II.


Accession: 003414057



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