Effects of temperature and duration of preservation of pig ovary on growth and survival of follicular oocytes in vitro

Hsu ShenFuh; Ju JyhCherng; Huang WeiTung; Tang PinChi; Liu BingTsan; Chiou ChiiMing; Yen SuChing; Wu MingFan; Cheng SanPao

Journal of the Chinese Society of Animal Science 29(2): 165-178


Accession: 003429335

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The objective of this study was to investigate the effect of the storage time and temperature on the survival rate and the developmental competence of oocytes from different follicular stages. Oocytes derived from the preantral follicles, antral follicles, cumulus-oocyte complexes and denuded oocytes were compared at 3 different storage temperatures (low temperature, 5-10 degrees C; room temperature, 20-25 degrees C; high temperature, 32-37 degrees C) with different duration of preservation (10, 30, or 60 min.). After 10-min. preservation, no significant difference was found among these four sources of oocytes derived from any group of ovaries stored at different temperatures. After a 30-min. preservation, the survival rate of the oocytes from the low temperature group was lower than that of groups stored at room temperature and high temperature (P<0.05). After 60-min. preservation, oocytes from the preantral follicles encapsulated with Ca-alginated gel had a significantly lower survival rate than the high temperature group after 4-days culture (53.5% vs. 76.5%, P<0.05). This was not the case for 30-min. preservation in which survival rates were 77.9 and 79.7%, respectively (P>0.05). No significant difference was found in the 3 different storage periods (10, 30, or 60 min.) for the oocytes derived from different categories of follicles (diameters: <3 mm, 3-5 mm, and 5-7 mm). However, when the follicles embedded with (1.5 micro g/ml) or without collagenase then cultured at 5-10 degrees C for 60 min. they displayed a higher survival rate than the 39 degrees C group (P<0.05). In conclusion, there was no immediate damage when the oocyte derived from different follicular stages were stored at the temperature of 5-10 degrees C. Transportation of pig ovaries from the slaughterhouse at 20-25 degrees C within 60 min. after slaughtered is recommended.