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Effects of vitrification medium composition on the survival of bovine in vitro produced embryos, following in straw-dilution, in vitro and in vivo following transfer

Pugh, P.A.; Tervit, H.R.; Niemann, H.

Animal Reproduction Science 58(1-2): 9-22

2000


ISSN/ISBN: 0378-4320
PMID: 10700641
DOI: 10.1016/s0378-4320(99)00087-1
Accession: 003429928

This study examined the effects of adding a macromolecule, polyvinylpyrrolidone (10% PVP) and a sugar (0.3 M trehalose) to vitrification solutions (VS) containing 1 (40% ethylene glycol, EG), 2 (25% EG + 25% dimethyl sulphoxide, DMSO) or 3 (20% EG + 20% DMSO + 10% 1.3-butanediol, BD) permeable cryoprotectants on the survival and hatching of in vitro produced cattle embryos, following vitrification, warming and in-straw cryoprotectant dilution.

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