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Electrophoretic test for efficiency of soil humic acids fractionation by gel chromatography and ultrafiltration

Electrophoretic test for efficiency of soil humic acids fractionation by gel chromatography and ultrafiltration

Biology Bulletin of the Russian Academy of Sciences 26(2): 170-173

Humic acids (HAs) extracted from chernozems with sodium pyrophosphate were fractionated by gel chromatography (GC) on a Sephadex G-75 column using 7 M urea as an eluant and ultrafiltration (UF) in the presence of 7 M urea or water. Chromatographic fractions and UF fractions from YM100, YM30, YM10, and YM5 membranes were analysed by polyacrylamide gel electrophoresis (PAGE).

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