EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice



Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice



Plant Physiology 125(3): 1508-1516



A major catabolic pathway for gibberellin (GA) is initiated by 2beta-hydroxylation, a reaction catalyzed by GA 2-oxidase. We have isolated and characterized a cDNA, designated Oryza sativa GA 2-oxidase 1 (OsGA2ox1) from rice (Oryza sativa L. cv Nipponbare) that encodes a GA 2-oxidase. The encoded protein, produced by heterologous expression in Escherichia coli, converted GA(1), GA(4), GA(9), GA(20), and GA(44) to the corresponding 2beta-hydroxylated products GA(8), GA(34), GA(51), GA(29), and GA(98), respectively. Ectopic expression of the OsGA2ox1 cDNA in transgenic rice inhibited stem elongation and the development of reproductive organs. These transgenic plants were deficient in endogenous GA(1). These results indicate that OsGA2ox1 encodes a GA 2-oxidase, which is functional not only in vitro but also in vivo. OsGA2ox1 was expressed in shoot apex and roots but not in leaves and stems. In situ hybridization analysis revealed that OsGA2ox1 mRNA was localized in a ring at the basal region of leaf primordia and young leaves. This ring-shaped expression around the shoot apex was drastically decreased after the phase transition from vegetative to reproductive growth. It was absent in the floral meristem, but it was still present in the lateral meristem that remained in the vegetative phase. These observations suggest that OsGA2ox1 controls the level of bioactive GAs in the shoot apical meristem; therefore, reduction in its expression may contribute to the early development of the inflorescence meristem.

(PDF emailed within 0-6 h: $19.90)

Accession: 003442671

Download citation: RISBibTeXText

PMID: 11244129

DOI: 10.1104/pp.125.3.1508



Related references

SHORT VEGETATIVE PHASE reduces gibberellin biosynthesis at the Arabidopsis shoot apex to regulate the floral transition. Proceedings of the National Academy of Sciences of the United States of America 111(26): E2760-9, 2014

Photo induced changes in rice m shoot apex part 1 changes in the shoot apex of a late winter rice m. New Phytologist 69(2): 347-353, 1970

Decreased shoot stature and grain l-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat. Journal of experimental botany 58(12): 3213-3226, 2007

Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat. Journal of Experimental Botany 58(12): 3213-3226, 2007

A rice homeobox gene, OSH1, alters hormonal contents and suppresses the gibberellin 20-oxidase gene expression in transgenic tobacco. Plant Physiology (Rockville) 114(3 SUPPL ): 161, 1997

Spatial patterns of cell cycle related gene expression in shoot apex of Arabidopsis. Journal of Experimental Botany 46(SUPPL ): 73, 1995

Studies in the differentiation of the shoot apex of rice. 2. The effect of the saline-alkali water irrigation upon the differentiation and the development of the shoot apex in rice.. Acta bot. sinica, 9: 1, 122-6. bibl. 3, 1960

Data on the ribosome content of the cells of the wheat shoot apex during the vegetative phase and the transition to the reproductive phase. Cytology and genetics3(4): 11-16, 1979

Some data on ribosome content in wheat shoot apex cells at the vegetative phase and during transition to the generative phase. TSitologiia i genetika 13(4): 252-257, 1979

Ribosome content in wheat triticum aestivum shoot apex cells in the vegetative phase and during transition to the generative phase. Tsitologiya i Genetika 13(4): 252-257, 1979

Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins. Journal of Plant Research 116(2): 161-164, 2003

Transcriptional, posttranscriptional, and posttranslational regulation of SHOOT MERISTEMLESS gene expression in Arabidopsis determines gene function in the shoot apex. Plant Physiology 167(2): 424-442, 2015

Photoinduced changes in rice shoot apex. 1. Changes in the shoot apex of a late-winter rice. New Phytologist, 69: 2, 347-353, 1970