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Expression of reporter genes from the defective RNA CD-61 of the coronavirus infectious bronchitis virus



Expression of reporter genes from the defective RNA CD-61 of the coronavirus infectious bronchitis virus



Journal of General Virology 81(Pt 7): 1687-1698



The defective RNA (D-RNA) CD-61, derived from the Beaudette strain of the avian coronavirus infectious bronchitis virus (IBV), was used as an RNA vector for the expression of two reporter genes, luciferase and chloramphenicol acetyltransferase (CAT). D-RNAs expressing the CAT gene were demonstrated to be capable of producing CAT protein in a helper-dependent expression system to about 1.6 micro g per 106 cells.

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Accession: 003442905

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PMID: 10859373


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