Impacts of RAPD amplification conditions on RAPD bands for Camellia

Yuan ChangChun; Shi SuHua; Ye ChuangXing

Journal of Tropical and Subtropical Botany 7(4): 313-317


Accession: 003470362

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The impacts of the concentrations of template DNA, primer, magnesium, dNTP and Taq DNA polymerase on RAPD amplification for Camellia assamica, C. sinensis and C. ptilophylla were tested by means of ladder analysis. RAPD band patterns were changed observably with the variation of concentrations of template DNA or primer or Taq DNA polymerase. The optimal amplification conditions for Camellia were chosen as follows: 4 ng template DNA/ micro l reaction volume, 15 ng primer/20 micro l reaction volume, 2.0 mmol MgCl2/litre, 200 micro mol dNTP/litre and 1 U Taq DNA polymerase/20 micro l reaction volume.