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Integration of Trypanosoma cruzi kDNA minicircle sequence in the host genome may be associated with autoimmune serum factors in Chagas disease patients



Integration of Trypanosoma cruzi kDNA minicircle sequence in the host genome may be associated with autoimmune serum factors in Chagas disease patients



Memorias do Instituto Oswaldo Cruz 94(Suppl. 1): 249-252



Integration of kDNA sequences within the genome of the host cell, shown by PCR amplification with primers to the conserved Trypanosoma cruzi kDNA minicircle sequence, was confirmed by Southern hybridization with specific probes. Cells containing the integrated kDNA sequences were perpetuated as transfected macrophage subclonal lines. The kDNA transfected macrophages expressed membrane antigens that were recognized by antibodies in a panel of sera from 10 patients with chronic Chagas' disease.

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Accession: 003480584

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DOI: 10.1590/s0074-02761999000700041


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