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Integrity of sperm plasm membrane, nucleus and mitochondria after freezing ram semen with ethylene glycol



Integrity of sperm plasm membrane, nucleus and mitochondria after freezing ram semen with ethylene glycol



Ciencia Rural 29(3): 527-531



Pools of semen from ejaculates collected from two rams were divided into two equal subsamples. One subsample was frozen with ethylene glycol and the other with glycerol. Sperm motility, vigour, acrosome status and membrane integrity were recorded for each sample. Sperm motility was evaluated in fresh semen, cooled semen, frozen semen, after 5 h at 38 degrees C or after 30 min at 45 degrees C.

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