+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation

Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation

Plant Journal 24(4): 429-435

Calcium-dependent protein kinases (CDPKs), the most abundant serine/threonine kinases in plants, are found in various subcellular localizations, which suggests that this family of kinases may be involved in multiple signal transduction pathways. A complete analysis to try to understand the molecular basis of the presence of CDPKs in various localizations in the cell has not been accomplished yet. It has been suggested that myristoylation may be responsible for membrane association of CDPKs. In this study, we used a rice CDPK, OSCPK2, which has a consensus sequence for myristoylation at the N-terminus, to address this question. We expressed wild-type OSCPK2 and various mutants in different heterologous systems to investigate the factors that affect its membrane association. The results show that OSCPK2 is myristoylated and palmitoylated and targeted to the membrane fraction. Both modifications are required, myristoylation being essential for membrane localization and palmitoylation for its full association. The fact that palmitoylation is a reversible modification may provide a mechanism for regulation of the subcellular localization. OSCPK2 is the first CDPK shown to be targeted to membranes by an src homology domain 4 (SH4) located at the N-terminus of the molecule.

(PDF emailed within 0-6 h: $19.90)

Accession: 003497091

Download citation: RISBibTeXText

PMID: 11115124

DOI: 10.1111/j.1365-313x.2000.00889.x

Related references

Chemical inhibition of myristoylation of the G-protein G-i1-alpha by 2-hydroxymyristate does not interfere with its palmitoylation or membrane association: Evidence that palmitoylation, but not myristoylation, regulates membrane attachment. Biochemical Journal 313(3): 717-720, 1996

The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families in Hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression. Febs Open Bio 7(1): 4-24, 2017

Calcium-dependent protein kinase (CDPK) and CDPK-related kinase (CRK) gene families in tomato: genome-wide identification and functional analyses in disease resistance. Molecular Genetics and Genomics 291(2): 661-676, 2016

Palmitoylation of a G protein ai subunit requires membrane localization not myristoylation. The Journal of Biological Chemistry 269: 898-903, 1994

Palmitoylation of a G protein alpha i subunit requires membrane localization not myristoylation. Journal of Biological Chemistry 269(49): 30898-30903, 1994

N-terminal myristoylation is required for membrane localization of cGMP-dependent protein kinase type II. Journal of Biological Chemistry 271(12): 7025-7029, 1996

The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. Journal of Biological Chemistry 288(20): 14332-14340, 2013

Palmitoylation-dependent membrane localization of the rice resistance protein pit is critical for the activation of the small GTPase OsRac1. Journal of Biological Chemistry 289(27): 19079-19088, 2014

BAALC 1-6-8 protein is targeted to postsynaptic lipid rafts by its N-terminal myristoylation and palmitoylation, and interacts with alpha, but not beta, subunit of Ca/calmodulin-dependent protein kinase II. Journal of Neurochemistry 92(3): 647-659, 2005

Synaptic localization of cyclic gmp dependent protein kinase type ii and the effect of n terminal myristoylation on its localization. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 644 13, 2002

14-3-3 Proteins activate a plant calcium-dependent protein kinase (CDPK). FEBS Letters 430(3): 381-384, 1998

1H, 15N and 13C assignments of the regulatory domains of calcium-dependent protein kinase (CDPK). Journal of Biomolecular Nmr 23(3): 249-250, 2002

A Calcium-dependent Protein Kinase (CDPK) in the Unicellular Green Alga Closterium ehrenbergii. Microbes and Environments 21(4): 278-283, 2006

Dynamic localization of calmodulin domain protein kinase (CDPK) and its relationship to calcium signalling in growing pollen tubes. Plant and Cell Physiology 46: S28-S28, 2005

Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation. Molecular and Biochemical Parasitology 184(2): 99-108, 2012