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Molecular cloning and sequence analysis of the VP7 gene of a Chinese isolate of bluetongue virus (strain Z1)

Molecular cloning and sequence analysis of the VP7 gene of a Chinese isolate of bluetongue virus (strain Z1)

Chinese Journal of Virology 16(1): 34-37

ISSN/ISBN: 1000-8721

Full length cDNA (1.5 kb) of the RNA segment encoding the major core protein VP7 of bluetongue virus (strain Z1) was amplified by RT-PCR. The amplified fragment was cloned into vector pGEM-T and was confirmed as the expected DNA by blue/white plaque screening, PCR test and EcoRI digestion. The DNA was further confirmed to be specific by Northern blotting using a DIG-labelled 1.15-kb fragment of the VP7 gene as probe.

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Accession: 003502384

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