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Obtaining a transgenic upland cotton harboring two insecticidal genes

Obtaining a transgenic upland cotton harboring two insecticidal genes

Acta Botanica Sinica 41(4): 384-388

pBinLK carrying two insecticidal genes, pea lectin (P-Lec) gene and soybean Kunitz trypsin inhibitor (SKTI) gene, was successfully transferred into 4 upland cotton (Gossypium hirsutum) cultivars (Xinluzao-1, Xinluzhong-2, Jihe-321 and Liao-9) via Agrobacterium-mediated transformation. After co-cultivation of hypocotyl segments with A. tumefaciens, kanamycin-resistant calli were screened, and somatic embryos and regenerated plants were obtained through various media.

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Accession: 003513294

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