Section 4
Chapter 3,519

PCR-ELISA for the CaMV-35S promoter as a screening method for genetically modified Roundup Ready soybeans

Brunnert, H.J.; Spener, F.; Borchers, T.

European Food Research and Technology 213(4/5): 366-371


ISSN/ISBN: 1438-2377
DOI: 10.1007/s002170100371
Accession: 003518134

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Screening of food components using a polymerase chain reaction (PCR) for the presence of genetic elements, such as the widespread cauliflower mosaic virus 35S promoter introduced into genetically modified organisms (GMOs), has become a routine method in modern food analysis. With the aim of developing a high throughput method suitable for automation, we established a PCR-enzyme-linked immunosorbent assay (PCR-ELISA).

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