PCR-ELISA for the CaMV-35S promoter as a screening method for genetically modified Roundup Ready soybeans
Brunnert, H.J.; Spener, F.; Borchers, T.
European Food Research and Technology 213(4/5): 366-371
2001
ISSN/ISBN: 1438-2377
DOI: 10.1007/s002170100371
Accession: 003518134
Screening of food components using a polymerase chain reaction (PCR) for the presence of genetic elements, such as the widespread cauliflower mosaic virus 35S promoter introduced into genetically modified organisms (GMOs), has become a routine method in modern food analysis. With the aim of developing a high throughput method suitable for automation, we established a PCR-enzyme-linked immunosorbent assay (PCR-ELISA).
PDF emailed within 0-6 h: $19.90
Related References
Kasama, K.; Watanabe, T.; Kikuchi, H.; Suzuki, T.; Tokishita, S.; Sakata, K.; Matsuki, A.; Hino, A.; Akiyama, H.; Maitani, T. 2005: Laboratory-performance study of the quantitative detection method for genetically modified soybeans (roundup ready soybeans 40-3-2) Shokuhin Eiseigaku Zasshi = Journal of the Food Hygienics Society of Japan 46(6): 270-276Kasama, K.; Watanabe, T.; Kikuchi, H.; Suzuki, T.; Tokishita, S.; Sakata, K.; Matsuki, A.; Hino, A.; Akiyama, H.; Maitani, T. 2005: Laboratory performance study of the quantitative detection method for genetically modified soybeans (roundup ready soybeans 40-3-2) Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan 46(6): 270-276
Jankiewicz, A.; Broll, H.; Zagon, J. 1999: The official method for the detection of genetically modified soybeans (German Food Act LMBG 35): a semi-quantitative study of sensitivity limits with glyphosate-tolerant soybeans (Roundup Ready) and insect-resistant Bt maize (Maximizer) European Food Research and Technology 209(2): 77-82
Fukuta, S.; Mizukami, Y.; Ishida, A.; Ueda, J.; Hasegawa, M.; Hayashi, I.; Hashimoto, M.; Kanbe, M. 2004: Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms European Food Research and Technology 218(5): 496-500
Ash, J.; Novak, C.; Scheideler, S.E. 2003: The fate of genetically modified protein from Roundup Ready Soybeans in laying hens Journal of Applied Poultry Research 12(2): 242-245
Xia, Y.; Chen, F.; Liu, K.; Zhang, L.; Duan, X.; Zhang, X.; Zhu, Z. 2019: Compositional differences between conventional Chinese and genetically modified Roundup Ready soybeans Crop and Pasture Science 70(6): 526-534
Nair, R.S.; Fuchs, R.L.; Schuette, S.A. 2002: Current methods for assessing safety of genetically modified crops as exemplified by data on Roundup Ready soybeans Toxicologic Pathology 30(1): 117-125
Ran, T.; Mei, L.; Lei, W.; Aihua, L.; Heru, H.R.; Jie, S. 2009: Detection of transgenic Dna in tilapias ( Oreochromis niloticus , Gift strain) fed genetically modified soybeans (Roundup Ready) Aquaculture Research 40(12): 1350-1357
Czerwinski, J.; Slupecka-Ziemilska, M.; Wolinski, J.; Barszcz, M.; Konieczka, P.; Smulikowska, S. 2015: The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 2. Functional status of the small intestine Journal of Animal and Feed Sciences 24(2): 144-152
Czerwinski, J.; Slupecka-Ziemilska, M.; Wolinski, J.; Barszcz, M.; Konieczka, P.; Smulikowska, S. 2015: The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 2. Functional status of the small intestine Journal of Animal and Feed Sciences 24(2): 144-152
Czerwinski, J.; Bogacki, M.; Jalali, B. M.; Konieczka, P.; Smulikowska, S. 2015: The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 1. Effects on performance and blood lymphocyte subpopulations Journal of Animal and Feed Sciences 24(2): 134-143
Czerwinski, J.; Bogacki, M.; Jalali, B. M.; Konieczka, P.; Smulikowska, S. 2015: The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 1. Effects on performance and blood lymphocyte subpopulations Journal of Animal and Feed Sciences 24(2): 134-143
Elodie Barbau-Piednoir; Antoon Lievens; Els Vandermassen; Etondoh-Guillaume Mbongolo-Mbella; Amaya Leunda-Casi; Nancy Roosens; Myriam Sneyers; Marc Van den Bulcke 2012: Four new SYBR®Green qPCR screening methods for the detection of Roundup Ready®, Liberty Link®, and CryIAb traits in genetically modified products European Food Research and Technology 234(1): 13-23
Hohne, M.; Santisi, C.R.; Meyer, R. 2002: Real-time multiplex PCR: An accurate method for the detection and quantification of 35S-CaMV promoter in genetically modified maize-containing food European Food Research and Technology 215(1): 59-64
Hagen, M.; Beneke, B. 2000: Detection of genetically modified soy (Roundup-Ready) in processed food products Berliner und Munchener Tierarztliche Wochenschrift 113(11-12): 454-458
Tozzini, A.C.; Martinez, M.C.; Lucca, M.F.; Vazquez Rovere, C.; Distefano, A.J.; Vas, M. del; Hopp, H.E. 2000: Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification EJB, Electronic Journal of Biotechnology 3(2): 1-5
Jang, H; Kwak, C Hwan; Kim, G; Kim, S Min; Huh, Y Suk; Jeon, T-Joon 2016: Identification of genetically modified DNA found in Roundup Ready soybean using gold nanoparticles Microchimica Acta 183(9): 2649-2654
Corkern, C.; Griffin, J.; Vidrine, P.; Miller, D.; Ellis, J.; Clay, P. 1999: Weed control in Roundup Ready soybeans with Roundup Ultra Proceedings Southern Weed Science Society 2(52): 51-52
Xu, C.; Li, L.; Jin, W.; Wan, Y. 2014: Recombinase polymerase amplification (RPA) of CaMV-35S promoter and nos terminator for rapid detection of genetically modified crops International Journal of Molecular Sciences 15(10): 18197-18205
Shao BiYing; Chen WenBing; Jiang ShuXun; Li ShouSong 2005: The detection of exogenous genes with duplex PCR in genetically modified soybean line Roundup Ready Journal of Fujian Agriculture and Forestry University Natural Science Edition 34(4): 483-487