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Physical mapping of 45S rRNA gene loci in the cucumber (Cucumis sativus L.) using fluorescence in situ hybridization



Physical mapping of 45S rRNA gene loci in the cucumber (Cucumis sativus L.) using fluorescence in situ hybridization



Caryologia 52(1/2): 49-57



Fluorescence in situ hybridization (FISH) using two different rDNA probes (18 S and 45 S) was applied to cucumber (Cucumis sativus) chromosomes. After counter-staining with 4'-6-diamidino-2-phenylindole (DAPI), all chromosomes could be identified based on the visible bands similar to C-banding patterns. The 18S and 45S rDNA signals appeared at the pericentromeric regions of chromosomes 1, 2 and 5.

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Accession: 003524556

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