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Production and characterization of rabbit sera for scrapie diagnosis

Production and characterization of rabbit sera for scrapie diagnosis

Selezione Veterinaria (Suppl): s153-s159

The aim was the standardization of a diagnostic method to detect the pathological isoform of PrP in scrapie. New rabbit sera were produced and characterized to use for detection of PrPSc by Western Blot. Sera were raised against a synthetic peptide corresponding to 97 to 111 residues of sheep PrP conjugated with a non-immunogenic poly-lysine core. Seven out of 8 sera recognized specifically the conjugated peptide as determined by ELISA reactions performed to assess sera titres.

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