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Quantitative determination of the major constituents of Verbena officinalis using high performance thin layer chromatography and high pressure liquid chromatography


Quantitative determination of the major constituents of Verbena officinalis using high performance thin layer chromatography and high pressure liquid chromatography



Phytochemical analysis: PCA 11(6): 351-355



ISSN/ISBN: 0958-0344

DOI: 10.1002/1099-1565(200011/12)11:6<351::aid-pca544>3.0.co;2-s

A pentacyclic triterpenoid, ursolic acid (1), two iridoid glucosides, verbenalin (2) and hastatoside (3), and a phenylpropanoid glycoside, verbascoside (4), were isolated from Verbena officinalis Linn. (Verbenaceae), a plant listed in the Chinese Pharmacopoeia and the British Herbal Pharmacopoeia. A procedure for the optimised extraction of these constituents for quantitative estimations has been established, An HPTLC method was employed for the determination of 1 using Liebermann Burchard reagent. A reversed-phase HPLC system with photodiode array detector was used to resolve compounds 2, 3 and 4 in the methanol extracts of different parts of the plant. Tender parts of the plant were rich in all of these constituents (0.24-0.34%, w/w) while the roots, which are not official in the Pharmacopoeias, contained a maximum amount (0.32%, w/w) of the bioactive verbascoside (4), The assay methods described are simple, rapid and accurate, and may form part of future drug authentication protocols.

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Accession: 003539619

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