+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Quantitative determination of the major constituents of Verbena officinalis using high performance thin layer chromatography and high pressure liquid chromatography

Quantitative determination of the major constituents of Verbena officinalis using high performance thin layer chromatography and high pressure liquid chromatography

Phytochemical analysis: PCA 11(6): 351-355

ISSN/ISBN: 0958-0344

DOI: 10.1002/1099-1565(200011/12)11:6<351::aid-pca544>;2-s

A pentacyclic triterpenoid, ursolic acid (1), two iridoid glucosides, verbenalin (2) and hastatoside (3), and a phenylpropanoid glycoside, verbascoside (4), were isolated from Verbena officinalis Linn. (Verbenaceae), a plant listed in the Chinese Pharmacopoeia and the British Herbal Pharmacopoeia. A procedure for the optimised extraction of these constituents for quantitative estimations has been established, An HPTLC method was employed for the determination of 1 using Liebermann Burchard reagent. A reversed-phase HPLC system with photodiode array detector was used to resolve compounds 2, 3 and 4 in the methanol extracts of different parts of the plant. Tender parts of the plant were rich in all of these constituents (0.24-0.34%, w/w) while the roots, which are not official in the Pharmacopoeias, contained a maximum amount (0.32%, w/w) of the bioactive verbascoside (4), The assay methods described are simple, rapid and accurate, and may form part of future drug authentication protocols.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003539619

Download citation: RISBibTeXText

Related references

Separation and quantitative determination of the principal pungent constituents of ginger zingiber officinale by a combination of thin layer chromatography and high pressure liquid chromatography. Planta Medica 39(3): 274, 1980

High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-advantages and limitations. Journal of Pharmaceutical and Biomedical Analysis 98: 52-59, 2014

Quantitative Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in Exforge Hct Tablets using High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography. Journal of Liquid Chromatography and Related Technologies 34(9-12): 981-994, 2011

Identification and determination of sulfonamides in tissues and serum of slaughter animals by high performance thin layer chromatography and high pressure liquid chromatography. Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung 176(1): 20-26, 1983

Quantitative determination of forskolin by thin layer chromatography and high pressure liquid chromatography. Planta Medica 1: 30-34, 1984

Quantitative determination of cucurbitacins by high performance liquid chromatography and high performance thin layer chromatography. Journal of Liquid Chromatography 16(2): 497-511, 1993

Determination of furanochromones and pyranocoumarins in drugs and Ammi visnaga fruits by combined solid-phase extraction-high-performance liquid chromatography and thin-layer chromatography-high-performance liquid chromatography. Journal of Chromatography. a 797(1-2): 305-309, 1998

Determination of zearalenone in cornflakes and other corn-based foods by thin layer chromatography, high pressure liquid chromatography, and gas-liquid chromatography/high resolution mass spectrometry. Journal - Association of Official Analytical Chemists 61(3): 593-600, 1978

Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using high-performance liquid chromatography. Pharmacognosy Magazine 8(30): 162-165, 2012

Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems. Journal of Chromatography A 886(1-2): 75-81, 2000

Qualitative and quantitative determination of sesquiterpenoids in Achillea species by reversed-phase high-performance liquid chromatography, mass-spectrometry and thin-layer chromatography. Journal of Chromatography. B Biomedical Sciences and Applications 729(1-2): 361-368, 1999

Identification and quantitative determination of priority nonionic and amphoteric surfactants in raw materials and detergent compositions by high-performance liquid chromatography, thin-layer chromatography, and UV spectrometry. Journal of Analytical Chemistry 61(7): 622-629, 2006

Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography. Advanced Pharmaceutical Bulletin 5(4): 587-591, 2015

High-performance thin-layer chromatographic determination of N-ethyl-3,4-methylenedioxyamphetamine and its major metabolites in urine and comparison with high-performance liquid chromatography. Journal of Chromatography. B Biomedical Sciences and Applications 688(1): 63-69, 1997

Comparison between high-performance thin-layer chromatography-fluorometry and high-performance liquid chromatography for the determination of sennosides A and B in senna (Cassia spp.) pods and leaves. Journal of Chromatography 303(2): 391-395, 1984