EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

RAPD characterisation of heterogeneity in spore progenies and sexuality in the genus Agaricus


RAPD characterisation of heterogeneity in spore progenies and sexuality in the genus Agaricus



Mycological Research 105(3): 370-376



ISSN/ISBN: 0953-7562

DOI: 10.1017/s0953756201003641

The genus Agaricus encompasses the cultivated mushroom A. bisporus, and includes both unifactorial heterothallic and homothallic species. The intractable nature of their life cycles and an absence of diagnostic morphological features, hinder analysis of breeding systems in Agaricus and some other homobasidiomycete species. We utilised RAPD markers to assess genetic variation in single spore progeny from different Agaricus species and to confirm heterokaryon formation in matings. Single spore progeny from heterothallic species should be more heterogeneous than those from homothallics. Homokaryotic progeny from the secondarily homothallic A. bisporus were less variable (12%) with fewer segregating loci than the known heterothallic species, A. bitorquis (50%) and A. nivescens (32%). Variation in the progeny of A. campestris (36%) was similar to that for the heterothallics. Heterothallism in the field mushroom was confirmed by RAPD analysis of putative matings; the first time A. campestris heterokaryons have been constructed. No variation was observed in progeny from two collections of A. subfloccosus. Homogeneity in RAPD profiles, absence of matings and self-fertility of single spores shows that A. subfloccosus is homothallic. In progeny from A. arvensis very low variation (5%) occurred and proportions of segregating loci were around half of those for A. bisporus. However, matings were observed and RAPD characterised within the A. arvensis progeny. RAPD analysis of genetic variation within spore progeny should have general utility in the characterisation of fungal breeding systems.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003540058

Download citation: RISBibTeXText

Related references

Sexuality in the genus Agaricus. Mushroom Science 1: 41-50, 1979

Genetics of sexuality in the genus Agaricus. Heredity 39(3): 431, 1977

Spore morphometry and the taxonomy of the genus agaricus. Ukrayins'kyi Botanichnyi Zhurnal 33(3): 246-251, 1976

Spore dimensions and taxonomy of the genus agaricus. Vestnik Moskovskogo Universiteta Seriya VI Biologiya Pochvovedenie 30(5): 50-54, 1975

Spore size and taxonomy of the genus Agaricus Fr. em. Karst. Moscow University Biological Sciences Bulletin 30(5-6): 36-39, 1975

The spore dimensions and taxonomy of the genus Agaricus Fr. en Karst. Vestnik Seriia VI Biologiia pochvovedenie: 5 50-54, 1975

The mitochondrial dna of agaricus heterogeneity in agaricus bitorquis and homogeneity in agaricus brunnescens. Current Genetics 9(2): 127-132, 1985

RAPD analysis of interspecific and intraspecific polymorphism of Agaricus bisporus and Agaricus bitorquis. Mycosystema 18(1): 55-60, 1999

Studies of the spore walls of Agaricus bisporus and Agaricus campestris. Canadian Journal of Microbiology 25(1): 32-39, 1979

The use of RAPD markers for characterisation of annual species of the genus Cicer. Annals of Agricultural Science Cairo 2(Special): 809-820, 2000

Systematic problems of the group Flavescentes of the genus Agaricus. i. agaricus augustus Fr. Mykologicky sbornik 52(2): 38-40, 1975

Taxonomic problems of the edible group of the genus Agaricus. iII. agaricus villaticus (Brond.) Hlav. and its strain. Mykologicky sbornik 53(5): 130-136, 1976

A new contribution to the knowledge of the genus Agaricus in Chile. Agaricus wrightii sp. nov. Mycotaxon 63: 71-76, 1997

Contribution to the knowledge of genus agaricus subgenus agaricus in mexico i. Revista Mexicana de Micologia 6: 151-177, 1990

Studies on the genus Agaricus L. : Fr. - the subgenus Agaricus section Sanguinolenti Schaeff. et Moller from north west India. Mushroom Research 6(2): 53-58, 1997