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Simultaneous determination of retinol acetate, retinol palmitate, cholecalciferol, alpha -tocopherol acetate and alphacalcidol in capsules by non-aqueous reversed-phase HPLC and column backflushing



Simultaneous determination of retinol acetate, retinol palmitate, cholecalciferol, alpha -tocopherol acetate and alphacalcidol in capsules by non-aqueous reversed-phase HPLC and column backflushing



Chromatographia 50(1/2): 109-112



A very simple non-aqueous reversed-phase HPLC method has been developed for analysis of retinol acetate, retinol palmitate, cholecalciferol, alpha -tocopherol acetate and alphacalcidol in capsules without the need for saponification. A reversed-phase (LiChrospher C8, 4.6 mm i.d.) column was used with acetonitrile-methanol, 95:5 (v/v) as mobile phase at a flow rate of 1 ml/min. Sample treatment consisted only of dilution of the capsule contents with n-hexane and methanol.

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Accession: 003560488

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