+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L



Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L



Plant Science 160(2): 301-313



Solanum aethiopicum is reported to carry resistance to bacterial wilt disease caused by Ralstonia solanacearum, which is one of the most important diseases of eggplant (Solanum melongena). These two species can sexually be crossed but the fertility of their progeny is very low. In order to transfer the resistance and improve the fertility, somatic hybrids between S. melongena cv. Dourga and two groups of S. aethiopicum were produced by electrical fusion of mesophyll protoplasts. Thirty hybrid plants were regenerated. When transferred to the greenhouse and transplanted in the field, they were vigorous and showed intermediate morphological traits. Their ploidy level was determined by DNA analysis through flow cytometry, and their hybrid nature was confirmed by examining isozymes and RAPDs patterns. Chloroplast DNA microsatellite analysis revealed that 18 hybrids had the chloroplasts of the eggplant and 12 those of the wild species. The parents and 16 hybrids were evaluated in the field for their fertility and resistance to bacterial wilt using a race 1, biovar 3 strain of R. solanacearum. All hybrids were fertile and set fruit with viable seeds. Their yield was either intermediate or as high as that of the cultivated eggplant. Both groups of S. aethiopicum were found tolerant to R. solanacearum, as about 50% of plants wilted after 8 weeks. The cultivated eggplant was susceptible with 100% of wilted plants 2 weeks after inoculation. All somatic hybrids tested were as tolerant as the wild species, except six hybrids showing a better level of resistance.

(PDF emailed within 0-6 h: $19.90)

Accession: 003563747

Download citation: RISBibTeXText

PMID: 11164602

DOI: 10.1016/s0168-9452(00)00394-0


Related references

Production and characterization of fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 85(6-7): 841-850, 1993

Fertile Solanum tuberosum + S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theoretical and Applied Genetics 98(8): 1272-1278, 1999

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae. Plant Cell Reports 20(11): 1022-1032, 2002

Genetic analysis of resistance to bacterial wilt (Ralstonia solanacearum) in eggplant (Solanum melongena L.). Acta Horticulturae Sinica 30(2): 163-166, 2003

ISSR and isozyme characterization of androgenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between Solanum melongena and Solanum aethiopicum group Gilo. Journal of Heredity 99(3): 304-315, 2008

A new source of resistance to bacterial wilt of eggplants obtained from a cross solanum aethiopicum x solanum melongena l. Agronomie 11(7): 555-560, 1991

On the origin of solanum melongena eggplant 1. cyto genetics of interspecific hybrids between solanum indicum and solanum surattense equals solanum xanthocarpum. Indian Journal of Botany 4(1): 91-98, 1981

Seed-borne nature of Ralstonia solanacearum in eggplant (Solanum melongena L.) cultivars in India. Seed Science and Technology 28(2): 291-299, 2000

Draft Genome Sequences of Nine Strains of Ralstonia solanacearum Differing in Virulence to Eggplant (Solanum melongena). Genome Announcements 4(1), 2016

Solanum phureja and S. stenotomum are sources of resistance to Ralstonia solanacearum for somatic hybrids of potato. Bacterial wilt disease and the Ralstonia solanacearum species complex: 253-259, 2005

Genetic diversity of Indian isolates of Ralstonia solanacearum causing bacterial wilt of eggplant (Solanum melongena). Indian Journal of Agricultural Sciences 87(11): 1466-1475, 2017

Transcriptome analysis and molecular marker discovery in Solanum incanum and S. aethiopicum, two close relatives of the common eggplant (Solanum melongena) with interest for breeding. Bmc Genomics 17: 300, 2016

Comparison of the resistance level of 9 solanum species to bacterial wilt pseudomonas solanacearum and root knot nematode meloidogyne incognita and possible implications in eggplant solanum melongena breeding for the hot humid tropics. Agronomie 5(1): 27-32, 1985

Comparison of the resistance level of nine Solanum species to bacterial wilt (Pseudomonas solanacearum) and root-knot nematode (Meloidogyne incognita) and possible implications in eggplant (Solanum melongena) breeding for the hot humid tropics. Agronomie: sciences des productions vegetales et de l'environnement(1): 27-32, 1985

Screening of elite eggplant (Solanum melongena) genotypes for bacterial wilt (Ralstonia solanacearum) in field conditions and their genetic association by using SSR markers. Indian Journal of Agricultural Sciences 88(10): 1502-1509, 2018