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Study on vector of Orientia tsutsugamushi and specimens of blood clots and spleen collected from artificial infected mice and field rodents by nested polymerase chain reaction


Study on vector of Orientia tsutsugamushi and specimens of blood clots and spleen collected from artificial infected mice and field rodents by nested polymerase chain reaction



Zhongguo Meijieshengwuxue ji Kongzhi Zazhi = Chinese Journal of Vector Biology and Control 11(3): 216-219



Nested PCR was used to detect O. tsutsugamushi DNA in Trombiculidae and samples collected from artificially infected mice and field rodents. Two pairs of primers were synthesized on the basis of the DNA sequence from Sta 58 kDa group-specific antigen gene. The products of PCR amplification were analysed by 2% agarose gel electrophoresis.

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