+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence



Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence



Journal of Virology 73(9): 7607-7618



Targeted recombination within the S (spike) gene of transmissible gastroenteritis coronavirus (TGEV) was promoted by passage of helper respiratory virus isolates in cells transfected with a TGEV-derived defective minigenome carrying the S gene from an enteric isolate. The minigenome was efficiently replicated in trans and packaged by the helper virus, leading to the formation of true recombinant and pseudorecombinant viruses containing the S proteins of both enteric and respiratory TGEV strains in their envelopes. The recombinants acquired an enteric tropism, and their analysis showed that they were generated by homologous recombination that implied a double crossover in the S gene resulting in replacement of most of the respiratory, attenuated strain S gene (nucleotides 96 to 3700) by the S gene of the enteric, virulent isolate. The recombinant virus was virulent and rapidly evolved in swine testis cells by the introduction of point mutations and in-phase codon deletions in a domain of the S gene (nucleotides 217 to 665) previously implicated in the tropism of TGEV. The helper virus, with an original respiratory tropism, was also found in the enteric tract, probably because pseudorecombinant viruses carrying the spike proteins from the respiratory strain and the enteric virus in their envelopes were formed. These results demonstrated that a change in the tropism and virulence of TGEV can be engineered by sequence changes in the S gene.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003576646

Download citation: RISBibTeXText

PMID: 10438851


Related references

Two amino acid changes at the N-terminus of transmissible gastroenteritis coronavirus spike protein result in the loss of enteric tropism. Virology 227(2): 378-388, 1997

Induction of an immune response to transmissible gastroenteritis coronavirus using vectors with enteric tropism. Advances in Experimental Medicine and Biology 342: 455-462, 1993

Induction of transmissible gastroenteritis coronavirus specific immune responses using vectors with enteric tropism. Advances in Experimental Medicine and Biology 371b: 1535-1541, 1995

The spike protein of transmissible gastroenteritis coronavirus controls the tropism of pseudorecombinant virions engineered using synthetic minigenomes. Advances in Experimental Medicine and Biology 440: 207-214, 1998

Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism. Journal of Virology 72(12): 9628-9636, 1998

Infection of porcine precision cut intestinal slices by transmissible gastroenteritis coronavirus demonstrates the importance of the spike protein for enterotropism of different virus strains. Veterinary Microbiology 205: 1-5, 2017

Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. Journal of Virology 77(16): 9084-9089, 2003

Cloning and sequence analysis of spike protein gene of swine transmissible gastroenteritis coronavirus. Journal of Northwest A and F University Natural Science Edition 35(7): 1-6, 2007

Cloning and sequence analysis of partial spike gene 5' region of transmissible gastroenteritis coronavirus TH-98 strain. Chinese Journal of Veterinary Medicine 40(4): 3-7, 2004

Evolution and tropism of transmissible gastroenteritis coronavirus. Advances in Experimental Medicine and Biology 342: 35-42, 1993

Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence. Virology 308(1): 13-22, 2003

Tropism of human adenovirus type 5-based vectors in swine and their ability to protect against transmissible gastroenteritis coronavirus. Journal of Virology 70(6): 3770-3780, 1996

Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Veterinary Quarterly 8(3): 257-261, 1986

Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus. Archives of Virology 145(8): 1725-1732, 2000

Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells. Virus Research 128(1-2): 52-57, 2007