The development of genetic tools for dissecting the biology of malaria parasites
de Koning-Ward, T.F.; Janse, C.J.; Waters, A.P.
Annual Review of Microbiology 54: 157-185
ISSN/ISBN: 0066-4227 PMID: 11018127 DOI: 10.1146/annurev.micro.54.1.157
This review describes the current status of genetic manipulation of Plasmodium parasites and the knowledge gained from the use of these systems, and discusses which genetic tools must still be developed. In the past decade, transfection systems have been developed for both human and animal model species of Plasmodium, providing a broad range of genetic tools. Transient transfection has been used to provide insight into the regulation of gene expression.