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Utilizing fowlpox virus recombinants to generate defective RNAs of the coronavirus infectious bronchitis virus



Utilizing fowlpox virus recombinants to generate defective RNAs of the coronavirus infectious bronchitis virus



Journal of General Virology 81(Pt 12): 2855-2865



Coronavirus defective RNAs (D-RNAs) have been used as RNA vectors for the expression of heterologous genes and as vehicles for reverse genetics by modifying coronavirus genomes by targeted recombination. D-RNAs based on the avian coronavirus infectious bronchitis virus (IBV) D-RNA CD-61 have been rescued (replicated and packaged into virions) in a helper virus-dependent manner following electroporation of in vitro-generated T7 transcripts into IBV-infected cells.

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Accession: 003607741

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PMID: 11086116


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