Validation of an isocratic HPLC method based on the use of ABZ+ Plus phase for the simultaneous determination of methylxantines, chlorogenic acid, some hydroxy-benzoic and hydroxy-cinnamic acids. Application to cocoa, coffee, tea and cola-drinks
Arlorio, M.; Coisson, J.D.; Martelli, A.
Chromatographia 52(9/10): 579-583
Validation of an isocratic HPLC method for determination of methylxantines (caffeine, theobromine, theophylline), chlorogenic acid and some hydroxy-benzoic/cinnamic acids (syringic, vanillic, phenolic, p-coumaric, ferulic and caffeic acids) in nervine foods and beverages is described. The method proposed can be usefully employed by single-wavelength/UV/HPLC-equipped Quality Control Laboratories.