+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A simple analytical procedure to replace HPLC for monitoring treatment concentrations of chloramine-T on fish culture facilities

A simple analytical procedure to replace HPLC for monitoring treatment concentrations of chloramine-T on fish culture facilities

Aquaculture 217(1-4): 61-72

Concentrations of chloramine-T must be monitored during experimental treatments of fish when studying the effectiveness of the drug for controlling bacterial gill disease. A surrogate analytical method for analysis of chloramine-T to replace the existing high-performance liquid chromatography (HPLC) method is described. A surrogate method was needed because the existing HPLC method is expensive, requires a specialist to use, and is not generally available at fish hatcheries. Criteria for selection of a replacement method included ease of use, analysis time, cost, safety, sensitivity, accuracy, and precision. The most promising approach was to use the determination of chlorine concentrations as an indicator of chloramine-T. Of the currently available methods for analysis of chlorine, the DPD (N,N-diethyl-p-phenylenediamine) colorimetric method best fit the established criteria. The surrogate method was evaluated under a variety of water quality conditions. Regression analysis of all DPD colorimetric analyses with the HPLC values produced a linear model (Y=0.9602 X+0.1259) with an r2 value of 0.9960. The average accuracy (percent recovery) of the DPD method relative to the HPLC method for the combined set of water quality data was 101.5%. The surrogate method was also evaluated with chloramine-T solutions that contained various concentrations of fish feed or selected densities of rainbow trout. When samples were analyzed within 2 h, the results of the surrogate method were consistent with those of the HPLC method. When samples with high concentrations of organic material were allowed to age more than 2 h before being analyzed, the DPD method seemed to be susceptible to interference, possibly from the development of other chloramine compounds. However, even after aging samples 6 h, the accuracy of the surrogate DPD method relative to the HPLC method was within the range of 80-120%. Based on the data comparing the two methods, the U.S. Food and Drug Administration has concluded that the DPD colorimetric method is appropriate to use to measure chloramine-T in water during pivotal efficacy trials designed to support the approval of chloramine-T for use in fish culture.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003627700

Download citation: RISBibTeXText

DOI: 10.1016/s0044-8486(02)00425-8

Related references

Drug monitoring and toxicology: a simple procedure for the monitoring of felbamate by HPLC-UV detection. Journal of Analytical Toxicology 32(5): 373-378, 2008

A Simple and Selective Analytical Procedure for the Extraction and Quantification of Lutein from Tomato By-Products by HPLC–DAD. Food Analytical Methods 5(4): 710-715, 2012

Simultaneous determination of antidepressant drugs and metabolites by HPLC. Design and validation of a simple and reliable analytical procedure. Biomedical Chromatography 8(4): 158-164, 1994

Two simple rapid methods for monitoring concentrations of theophylline in human serum by HPLC. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae 9(1): 55-59, 1987

Monitoring and effects of nicosulfuron in aquatic mesocosms: development of a simple analytical procedure and evidence for low toxicity to phytoplankton communities. Analytical and Bioanalytical Chemistry 382(1): 108-114, 2005

HPLC determination of plasma thiocyanate concentrations in fish blood: application to laboratory pharmacokinetic and field-monitoring studies. Ecotoxicology and Environmental Safety 30(3): 302-308, 1995

HPLC-based procedure for the preparation of carbene-generating photoreactive GM3 and GM1 ganglioside derivatives radioiodinated to high specific radioactivity with chloramine T as an oxidant. Analytical Biochemistry 340(2): 373-375, 2005

Can HPLC replace GC as a viable, accurate, routine procedure for determining carbohydrates in sugar cane juices and factory products?. Proceedings of the sugar processing research conference: 91-197, 1992

Analytical procedure for determination of S-adenosylmethionine, S-adenosylhomocysteine, and S-adenosylethionine in same isocratic HPLC run, with a procedure for preparation and analysis of the analog S-adenosylhomocysteine sulfoxide. Journal Of Liquid Chromatography. 18(10): 2005-2017, 1995

Reversed-phase HPLC. Analytical procedure. Methods in Molecular Biology 36: 23-35, 1994

Drug monitoring and toxicology: a procedure for the monitoring of oxcarbazepine metabolite by HPLC-UV. Journal of Chromatographic Science 44(1): 45-48, 2006

Drug monitoring and toxicology: a procedure for the monitoring of levetiracetam and zonisamide by HPLC-UV. Journal of Analytical Toxicology 30(1): 27-30, 2006

A novel green analytical procedure for monitoring of azoxystrobin in water samples by a flow injection chemiluminescence method with off-line ultrasonic treatment. Luminescence 28(5): 641-647, 2014