Additive action of vitamins C and e against hydrocortisone-induced genotoxicity in human lymphocyte chromosomes
Ahmad, S.; Hoda, A.; Afzal, M.
International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 72(4): 204-209
ISSN/ISBN: 0300-9831 PMID: 12214557 DOI: 10.1024/0300-98184.108.40.206
In earlier reports, hydrocortisone administration to human lymphocytes in culture was shown to cause chromosomal aberrations and increased sister chromatid exchanges. With a view to study the ameliorative action of some antioxidants against this effect, vitamins C and E were used separately and in combination along with hydrocortisone treatment, at different dosage and for different durations, on human lymphocyte cultures. The levels of chromosomal aberrations and sister chromatid exchanges were lowered, suggesting a protective role of vitamins against genotoxic damage. Administration of vitamins C and E combined appeared to be more effective in preventing chromosomal damage than separate administration, demonstrating the additive action of these vitamins against steroid-induced genotoxicity.