+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses



Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses



Journal of General Virology 83(1): 229-239



An isolate of Strawberry mottle virus (SMoV) was transferred from Fragaria vesca to Nicotiana occidentalis and Chenopodium quinoa by mechanical inoculation. Electron micrographs of infected tissues showed the presence of isometric particles of approximately 28 nm in diameter. SMoV-associated tubular structures were also conspicuous, particularly in the plasmodesmata of C. quinoa. DsRNA extraction of SMoV-infected N. occidentalis yielded two bands of 6.3 and 7.8 kbp which were cloned and sequenced. Gaps in the sequence, including the 5' and 3' ends, were filled using RT-PCR and RACE. The genome of SMoV was found to consist of RNA1 and RNA2 of 7036 and 5619 nt, respectively, excluding a poly(A) tail. Each RNA encodes one polyprotein and has a 3' non-coding region of approximately 1150 nt. The polyprotein of RNA1 contains regions with identities to helicase, viral genome-linked protein, protease and polymerase (RdRp), and shares its closest similarity with RNA1 of the tentative nepovirus Satsuma dwarf virus (SDV). The polyprotein of RNA2 displayed some similarity to the large coat protein domain of SDV and related viruses. Phylogenetic analysis of the RdRp region showed that SMoV falls into a separate group containing SDV, Apple latent spherical virus, Naval orange infectious mottling virus and Rice tungro spherical virus. Given the size of RNA2 and the presence of a long 3' non-coding region, SMoV is more typical of a nepovirus, although atypically for a nepovirus it is aphid transmissible. We propose that SMoV is a tentative member of an SDV-like lineage of picorna-like viruses.

(PDF emailed within 0-6 h: $19.90)

Accession: 003674341

Download citation: RISBibTeXText

DOI: 10.1099/0022-1317-83-1-229


Related references

Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses. Journal of General Virology 83(Pt 1): 229-239, 2001

Complete nucleotide sequence of clematis chlorotic mottle virus, a new member of the family Tombusviridae. Archives of Virology 162(5): 1373-1379, 2017

Complete nucleotide sequence of Oat necrotic mottle virus: a distinct Tritimovirus species (family Potyviridae) most closely related to Wheat streak mosaic virus. Archives of Virology 149(3): 633-640, 2004

Complete nucleotide sequence and genome organization of Calibrachoa mottle virus - a new species in the genus Carmovirus of the family Tombusviridae. Virus Research7: 2, 216-223, 2010

Complete nucleotide sequence of jasmine virus H, a new member of the family Tombusviridae. Archives of Virology: -, 2017

Complete nucleotide sequence and genome organization of Calibrachoa mottle virus (CbMV)--a new species in the genus Carmovirus of the family Tombusviridae. Virus Research 147(2): 216-223, 2010

Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae. Archives of Virology 158(9): 1917-1923, 2013

Complete nucleotide sequence of rose yellow leaf virus, a new member of the family Tombusviridae. Archives of Virology 159(10): 2795-2798, 2014

Complete nucleotide sequence of Rosa rugosa leaf distortion virus, a new member of the family Tombusviridae. Archives of Virology 158(12): 2617-2620, 2014

Complete Nucleotide Sequence of Artichoke latent virus Shows it to be a Member of the Genus Macluravirus in the Family Potyviridae. Phytopathology 105(8): 1155-1160, 2015

Complete nucleotide sequence and taxonomy of Sugarcane streak mosaic virus, member of a novel genus in the family Potyviridae. Virus Genes 40(3): 432-439, 2010

Complete nucleotide sequence of rose yellow vein virus, a member of the family Caulimoviridae having a novel genome organization. Archives of Virology 158(4): 877-880, 2013

Complete nucleotide sequence and genome organization of Olive latent virus 3, a new putative member of the family Tymoviridae. Virus Research 152(1-2): 10-18, 2010

The nucleotide sequence of parsnip yellow fleck virus: a plant picorna-like virus. Journal of General Virology 73: 3203-3211, 1992

Complete Nucleotide Sequence of a French Isolate of Maize rough dwarf virus, a Fijivirus Member in the Family Reoviridae. Genome Announcements 4(4): -, 2016