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Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay



Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay



Clinical and Diagnostic Laboratory Immunology 9(6): 1343-1347



A Baculovirus carrying the surface antigen 2 (SAG2) gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs infected with T. gondii. Mice immunized with S-rSAG2 produced high titres of specific immunoglobulin G2a (IgG2a) and IgG1 antibodies.

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Accession: 003674794

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PMID: 12414772

DOI: 10.1128/CDLI.9.6.1343-1347.2002


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